Template switching protocol and type of biases in NGS. (A) Schematic
Template Switching Oligo. Web this 5′ race protocol contains two steps. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse.
Template switching protocol and type of biases in NGS. (A) Schematic
In the first step, template switching reverse transcription reaction. Web the cdna synthesis and amplification protocol contains two steps. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse. In the first step, cdna is synthesized using. Web this 5′ race protocol contains two steps.
Web this 5′ race protocol contains two steps. In the first step, cdna is synthesized using. In the first step, template switching reverse transcription reaction. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse. Web the cdna synthesis and amplification protocol contains two steps. Web this 5′ race protocol contains two steps.
